Czech J. Genet. Plant Breed., 2015, 51(3):96-109 | DOI: 10.17221/205/2014-CJGPB

Agro-morphological and molecular diversity in castor (Ricinus communis L.) germplasm collected from Andaman and Nicobar Islands, IndiaOriginal Paper

Meena KANTI1, Kammili ANJANI2, Betha USHA KIRAN2, K. VIVEKANANDA2
1 Central Research Institute for Jute and Allied Fibres, Barrackpore, India
2 Directorate of Oilseeds Research, Hyderabad, India

Castor (Ricinus communis L.) is an industrial oilseed crop grown worldwide. Its oil with more than 80% ricinoleic acid makes it a chief raw material for numerous industrial applications and biofuel production. Castor grows in a wild form across India including Andaman & Nicobar Islands, which are geographically quite isolated from mainland India. Thirty-three accessions growing in isolation in these Islands were used in the present study. Genetic diversity among these accessions was assessed using 18 agro-morphological traits and 29 EST-SSR markers. High agro-morphological and molecular variability was observed among these accessions. Both agro-morphological traits and EST-SSR markers effectively discriminated the accessions. However, EST-SSRs separated the accessions into more groups than did agro-morphological data, implying high efficiency and resolution of EST-SSR markers in genetic analysis of castor germplasm from Andaman & Nicobar. The diverse accessions identified in the present investigation would serve as genetically diverse sources in castor breeding programmes.

Keywords: agro-morphological traits; EST-SSRs; genetic diversity; polymorphism

Published: September 30, 2015  Show citation

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KANTI M, ANJANI K, USHA KIRAN B, VIVEKANANDA K. Agro-morphological and molecular diversity in castor (Ricinus communis L.) germplasm collected from Andaman and Nicobar Islands, India. Czech J. Genet. Plant Breed.. 2015;51(3):96-109. doi: 10.17221/205/2014-CJGPB.
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References

  1. Allan G., Williams A., Rabinowicz P.D., Chan A.P., Ravel J., Keim P. (2008): Worldwide genotyping of castor bean germplasm (Ricinus communis L.) using AFLP and SSRs. Genetic Resources and Crop Evolution, 55: 365-378. Go to original source...
  2. Anderson J.A., Churchill G.A., Autroque J.E., Tanksley S.D., Swells M.E. (1993): Optimizing selection for plant linkage map. Genome, 36: 181-186. Go to original source... Go to PubMed...
  3. Anjani K. (2001a): Exploration of castor (Ricinus communis L.) germplasm in Andaman and Nicobar Islands. IPGRI Newsletter for Asia, the Pacific and Oceania, 34: 10.
  4. Anjani K. (2001b): Catalogue of Castor (Ricinus communis L.) (1990-2000). Vol. 1. Hyderabad, Germaplasm Management Unit (Castor), Directorate of Oilseeds Research.
  5. Anjani K. (2010): Extra-early maturing germplasm for utilization in castor improvement. Industrial Crops and Products, 31: 139-144 Go to original source...
  6. Anjani K. (2012): Castor genetic resources: A primary gene pool for exploitation. Industrial Crops and Products, 35: 1-4. Go to original source...
  7. Athma P., Vaidyanath K., Reddy T.P. (1982): Peroxidase isoenzyme studies in certain varieties of Ricinus communis L. Indian Journal of Botany, 5: 178-182.
  8. Bajay M.M., Pinheiro J.D., Batista C.E.A., Nobrega M.B.M., Zucchi M.I. (2009): Development and characterization of microsatellite markers for castor (Ricinus communis L.), an important oleaginous species for biodiesel production. Conservation Genetics and Resources, 1: 237-239. Go to original source...
  9. Bajay M.M., Zucchi M.I., Kiihl T.A.M., Batista C.E.A., Monteiro M., Pinheiro J.B. (2011): Development of a novel set of microsatellite markers for Castor bean, Ricinus communis (Euphorbiaceae). American Journal of Botany, 98: E87-E89. Go to original source... Go to PubMed...
  10. Berman P., Nizri S., Wiesman Z. (2011): Castor oil biodiesel and its blends as alternative fuel. Biomass and Bioenergy, 35: 2861-2866. Go to original source...
  11. Birchler J.A., Auger D.L., Riddle N.C. (2003): In search of the molecular basis of heterosis. Plant Cell, 15: 2236-2239. Go to original source... Go to PubMed...
  12. Brigham R.D. (1967): Natural out crossing in dwarf internode castor, Ricinus communis L. Crop Science, 7: 353-355. Go to original source...
  13. Chan A.P., Crabtree J., Zhao Q., Lorenzi H., Orvis J., Puiu D., PIC > 0.5 can also be useful for a wide range of genetic Melake-Berhan A., Jones K.M., Redman J., Chen G., investigations such as association mapping, linkage map. Highly diverse accessions identified based on agro-morphological and molecular studies can be utilized in hybrid development programmes.
  14. Cahoon E.B., Gedil M., Stanke M., Haas B.J., Wortman
  15. J.R., Fraser-Liggett C.M., Ravel J., Rabinowicz P.D. (2010): Draftgenome sequence of the oilseed species Ricinus communis. Nature Biotechnology, 28: 951-956. Go to original source... Go to PubMed...
  16. Costa M.N., Pereira W.E., Bruno R.L.A, Freire E.C., Noberega M.B.M., Milani M., Oliveira A.P. (2006): Genetic divergence between castor bean accessions and cultivars through multivariate statistics. Pesquisa Agropecuária Brasileira, 41: 1617-1622. Go to original source...
  17. Demirbas A. (2007): Importance of biodiesel as transportation fuel. Energy Policy, 35: 4661-4670. Go to original source...
  18. Doyle J.J., Doyle J.L. (1990): Isolation of plant DNA from fresh tissue. Focus, 12: 13-15. Go to original source...
  19. Esselman E.J., Crawford D.J., Brauner S., Stuessy T.F., Anderson G.J., Silva O.M. (2000): RAPD marker diversity within and divergence among species of Dendroseris (Asteraceae: Lactuceae). American Journal of Botany, 87: 591-596. Go to original source...
  20. Feng S., Li W., Huang H., Wang J., Wu Y. (2009): Development, characterization and cross-species/genera transferability of EST-SSR markers for rubber tree (Hevea brasiliensis). Molecular Breeding, 23: 85-97. Go to original source...
  21. Figueiredo Neto A., Almeida F.A.C., Gouveia Jpg D.E., Nobrega M.B.M., Carneiro R.M., Pedroza J.P. (2004): Genetic divergence in accessions of castor bean (Ricinus communis L.) based on characteristics of seed. Journal of Biology and Earth Sciences, 4: 1-10.
  22. Foster J.T., Allan G.J., Chan A.P., Rabinowicz P.D., Ravel J., Jackson P.J., Keim P. (2010): Single nucleotide polymorphisms for assessing genetic diversity in castor bean (Ricinus communis). BMC Plant Biology, 10: 13-22. Go to original source... Go to PubMed...
  23. Gaginella T.S., Capasso F., Mascolo N., Perilli S. (1998): Castor oil: new lessons from an ancient oil. Phytotherapy Research, 12(S1): S128-S130. Go to original source...
  24. Gajera B.B., Kumar N., Amritpal S.S., Bhupendra S.P., Ravikiran R., Subhash N., Jadeja G.C. (2010): Assessment of genetic diversity in castor (Ricinus communis L.) using RAPD and ISSR markers. Industrial Crops and Products, 32: 491-498. Go to original source...
  25. Gogoi S.N., Sarma R.N., Rajan R.K. (2011): Genetic diversity of castor (Ricinus communis L.) germplasm in North-eastern region of India. Indian Journal of Sericulture, 50: 51-57.
  26. Govaerts R., Frodin D.G, Radcliffe-Smith A. (2000): World Cheklist and Bibliography of Euphorbiaceae (with Pandaceae). Trowbridge, Redwood Books, Ltd.
  27. Jain S., Jain R.K., Mccouch S.R. (2004): Genetic analysis of Indian aromatic and quality rice (Oryza sativa L.) germplasm using panels of fluorescently-labeled microsatellite markers. Theoretical and Applied Genetics, 109: 965-977. Go to original source... Go to PubMed...
  28. Jeong G.T., Park D.H. (2009): Optimization of biodiesel production from castor oil using response surface methodology. Applied Biochemistry and Biotechnology, 156: 431-441. Go to original source... Go to PubMed...
  29. Kaiser H.F. (1960): The application of electronic computers to factor analysis. Educational and Psychological Measurement, 20: 141-151. Go to original source...
  30. Kanti M., Anjani K., Venkatramya K. (2014): Molecular Diversity in Castor Germplasm Collection Originated from North-Eastern Hill Province of India. International Journal Research for Scientific Innovations, 1: 1-6.
  31. Kupper C.P.J.M., Chiotatto A., Zucchi M., Colombo C., Carbonell S., Costa M.J.M., Gazaffi R., Franco G.A.A., De Campos T., De Souza A. (2011): Genetic diversity in cultivated carioca common beans based on molecular marker analysis. Genetics and Molecular Biology, 34: 88. Go to original source... Go to PubMed...
  32. Lowrey T.K., Crawford D.J. (1985): Allozyme divergence and evolution in Tertramolopium (Compositae: Astereae) on the Hawaiian Islands. Systematic Botany, 10: 64-72. Go to original source...
  33. Meinders H.C., Jones M.D. (1950): Pollen shedding and dispersalin the castor plant Ricinus communis L. Journal of Agronomy, 4: 206-209. Go to original source...
  34. Milani M., Dantas F.V., Martins W.F.S. (2009): Genetic divergence among castor bean genotypes by morphologic and molecular characters. Revista Brasileira de Oleaginosas e Fibrosas, 13: 61-71.
  35. Moshkin V.A (ed.) (1986): Castor. New Delhi, Amerind Publishing Co., Pvt. Ltd.
  36. Ogunniyi D.S. (2006): Castor oil: a vital industrial raw material. Bioresource Technology, 97: 1086-1091. Go to original source... Go to PubMed...
  37. Perrier X., Flori A., Bonnet F. (2003): Data analysis methods. In: Hamon P., Seguin M., Perrier X., Glaszmann J.C. (eds): Genetic Diversity of Cultivated Tropical Plants. Montpellier, Enfield, Science Publisher: 43-76.
  38. Pinzi S., Garcia I.L., Lopez-Gimenez F.J., Castro M.D.L., Dorado G., Dorado M.P. (2009): The ideal vegetable oilbased biodiesel composition: a review of social, economical and technical implications. Energy Fuels, 23: 2325-2341. Go to original source...
  39. Qiu L., Yang C., Tian B., Yang J.B., Liu A. (2010): Exploiting EST database for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.) BMC Plant Biology, 10: 278-287. Go to original source... Go to PubMed...
  40. Reif J.C., Gumpert F.M., Fischer S., Melchinger A.E. (2007): Impact of inter population divergence on additive and dominance variance in hybrid populations. Genetics, 176: 1931-1934. Go to original source... Go to PubMed...
  41. Rivarola M., Foster J.T., Chan A.P., Williams A.L., Rice D.W., Liu X., Melake-Berhan A., Creasy H.H., Puiu D., Rosovitz M.J., Khouri H.M., Beckstrom-Sternberg S.M., Allan G.J., Keim P., Ravel J., Rabinowicz P.D. (2011): Castor bean organelle genome sequencing and worldwide genetic diversity analysis. PLoS ONE, 6: e21743. Go to original source... Go to PubMed...
  42. Santalla M., Power J.B., Davey M.R. (1998): Genetic diversity in mung bean germplasm revealed by RAPD markers. Plant Breeding, 117: 473-478. Go to original source...
  43. Sathaiah V., Reddy T.P. (1983): Inheritance of isoperoxidases in castor. In: 15th Int. Congress of Genetics, Dec 12-21, 1983, New Delhi, Abstracts Part 1: 387.
  44. Sathaiah V., Reddy T.P. (1984): Qualitative and quantitative analysis of peroxidase and esterase enzymes in castor (Ricinus communis L.). Indian Journal of Botany, 7: 68-73.
  45. Sathaiah V., Reddy T.P. (1988): Esterase isozyme patterns of nine parental diallel set of castor (Ricinus communis L.). In: Proc. Conf. Cytology Genetics, 1: 240-246.
  46. Sayama T., Hwang T.Y., Komatsu K., Takada Y., Takahashi M., Kato S., Sasama H., Higashi A., Nakamoto Y., Funatsuki H., Ishimoto M. (2011): Development and application of a whole-genome simple sequence repeat panel for high-through put genotyping in soybean. DNA Research, 18: 107-115. Go to original source... Go to PubMed...
  47. Scholz V., Silva J.N. (2008): Prospects and risks of the use of castor oil as a fuel. Biomass and Bioenergy, 32: 95-100. Go to original source...
  48. Singh A.K. (2011). Castor oil-based lubricant reduces smoke emission in two-stroke engines. Industrial Crops and Products, 33: 287-295. Go to original source...
  49. Soltis P.S., Soltis D.E., Tucker T.L., Lang A. (1992): Allozyme variability is absent in the narrow endemic Bensoniellaoregona (Saifragacear). Conservation Biology, 6: 131-134. Go to original source...
  50. Talia P., Nishinakamasu V., Hopp H.E., Heinz R.A. Paniego N. (2010): Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map. Electronic Journal of Biotechnology, 13: 6. Go to original source...
  51. Tessier C., David J., This P., Boursiquot J.M., Charrier A. (1999): Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L. Theoretical and Applied Genetics, 98: 171-177. Go to original source...
  52. Vivodík M., Balážová Z., Gálová Z. (2014): RAPD analysis of genetic diversity of castor bean. International Journal of Biological, Veterinary, Agricultural and Food Engineering, 8: 615-618.
  53. Ward J.H. Jr. (1963): Hierarchical grouping to optimize an objective function. Journal of American Statistical Association, 58: 236-244. Go to original source...
  54. Williams J.G.K., Kubelik A.R., Livak K.J., Rafalsky A., Tingey S.V. (1990): DNA polymorphisms amplified by arbitary primers are useful as genetic markers. Nucleic Acids Research, 18: 6531-6535. Go to original source... Go to PubMed...

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